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李茹萍
摘要 在台湾，蚬为养殖的淡水双壳类之最大宗。此物以前多用于肝炎治疗，近期报告则指出，蚬具有多
种生物活性，但其是否会影响炎症仍属未知。本研究探讨蚬的抗炎症活性。 首先，将蚬以氯仿处理，获得
蚬的氯仿萃取物（CCES），供体内及体外试验。结果显示CCES 显著降低脂多醣（LPS）所引起的促发炎
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•295•
实 验 研 究
Experimental study
细胞因子的释放。此外，CCES 降低LPS 所引起的脏器损伤。实时聚合酶链反应(real-time PCR)分析显示
CCES 有抑制LPS 所引起的介白质-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）之mRNA 表现。 西方墨点法
(Western blot)分析发现CCES 增加IκB 及减少其磷酸化。气相色层－质谱分析则建议CCES 里的植物固醇
和脂肪酸为具有抗发炎的活性。综观上述结果，可见CCES 有开发为抗发炎功能性食品的潜力。
关键词 蚬、IL-1β、炎症、IκB-α、TNF-α
Inhibitory Effects of Chloroform Extracts Derived from Corbicula fluminea on the Release of
Pro-inflammatory Cytokines
Ru-Ping Lee
ABSTRACT Corbicula fluminea, the primary freshwater bivalve cultivated in Taiwan, was formerly used as a
remedy for hepatitis. Recent reports indicate that C. fluminea has many bioactivities, but it remains unknown
whether C. fluminea affects inflammation. This study explored the anti-inflammatory activity of C. fluminea. C.
fluminea was first treated with chloroform to obtain clam chloroform extracts (CCEs). On the basis of the assay
for the release of pro-inflammatory cytokines in vitro and in vivo, the results show that the CCEs significantly
lowered the release of lipopolysaccharide (LPS)-induced pro-inflammatory cytokines. Additionally, the CCEs
reduced LPS-induced organ damage. Real-time polymerase chain reaction analysis suggested that CCEs inhibit
the LPS-induced mRNA expression of interleukin-1β and tumor necrosis factor-α. Western blot analysis indicated
that the CCEs increased expression of IκB and attenuated the phosphorylation of IκB. Gas chromatography−mass
spectrometry suggests that phytosterols and fatty acids are responsible for the anti-inflammatory properties of
CCEs. Taken together, CCEs have the potential to be developed as an anti-inflammatory functional food.
KEYWORDS: Freshwater clam, IL-1β, inflammation, IκB-α, TNF-α
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•29 6•
针 灸 经 络
Acupuncture & meridians
摘要 在台湾，蚬为养殖的淡水双壳类之最大宗。此物以前多用于肝炎治疗，近期报告则指出，蚬具有多
种生物活性，但其是否会影响炎症仍属未知。本研究探讨蚬的抗炎症活性。 首先，将蚬以氯仿处理，获得
蚬的氯仿萃取物（CCES），供体内及体外试验。结果显示CCES 显著降低脂多醣（LPS）所引起的促发炎
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•295•
实 验 研 究
Experimental study
细胞因子的释放。此外，CCES 降低LPS 所引起的脏器损伤。实时聚合酶链反应(real-time PCR)分析显示
CCES 有抑制LPS 所引起的介白质-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）之mRNA 表现。 西方墨点法
(Western blot)分析发现CCES 增加IκB 及减少其磷酸化。气相色层－质谱分析则建议CCES 里的植物固醇
和脂肪酸为具有抗发炎的活性。综观上述结果，可见CCES 有开发为抗发炎功能性食品的潜力。
关键词 蚬、IL-1β、炎症、IκB-α、TNF-α
Inhibitory Effects of Chloroform Extracts Derived from Corbicula fluminea on the Release of
Pro-inflammatory Cytokines
Ru-Ping Lee
ABSTRACT Corbicula fluminea, the primary freshwater bivalve cultivated in Taiwan, was formerly used as a
remedy for hepatitis. Recent reports indicate that C. fluminea has many bioactivities, but it remains unknown
whether C. fluminea affects inflammation. This study explored the anti-inflammatory activity of C. fluminea. C.
fluminea was first treated with chloroform to obtain clam chloroform extracts (CCEs). On the basis of the assay
for the release of pro-inflammatory cytokines in vitro and in vivo, the results show that the CCEs significantly
lowered the release of lipopolysaccharide (LPS)-induced pro-inflammatory cytokines. Additionally, the CCEs
reduced LPS-induced organ damage. Real-time polymerase chain reaction analysis suggested that CCEs inhibit
the LPS-induced mRNA expression of interleukin-1β and tumor necrosis factor-α. Western blot analysis indicated
that the CCEs increased expression of IκB and attenuated the phosphorylation of IκB. Gas chromatography−mass
spectrometry suggests that phytosterols and fatty acids are responsible for the anti-inflammatory properties of
CCEs. Taken together, CCEs have the potential to be developed as an anti-inflammatory functional food.
KEYWORDS: Freshwater clam, IL-1β, inflammation, IκB-α, TNF-α
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•29 6•
针 灸 经 络
Acupuncture & meridians
