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吉 勤 李 潇 杨文荣 谷 艳 谢江海
（云南省中医医院，云南昆明，650021）
摘要 目的：观察降浊颗粒对5/6 肾切除慢性肾衰竭大鼠肾组织α- 平滑肌肌动蛋白（α- ***A)的影响，以
观察降浊颗粒对慢性肾衰竭(CRF)的治疗作用及抗肾间质纤维化的机理。方法： 5/6 肾大部切除法（两期
手术）建立CFR 大鼠模型。动物灌胃给生理盐水或给药连续60 天。取大鼠肾脏病理切片，采用免疫组织
化学染色（SP 法）法检测α-***A 表达情况。结果：降浊颗粒能够降低实验大鼠肾组织中的α-***A 的表
达，与模型对照组比较差异有显著性意义（P<0.05），与贝那普利对照组比较差异无显著性（P>0.05）。
结论：降浊颗粒可以抑制大鼠肾组织α-***A 的表达，干预肾间质纤维化，从而延缓大鼠CRF 进展，其疗
效与贝那普利相当。
关键词 降浊颗粒；慢性肾衰竭； 肾间质纤维化；α- 平滑肌肌动蛋白
The Influence of Turbidity-reducing Granule on α- ***A of Rats with Chronic Renal Failure
Ji qin Li xiao Yang wen－rong Gu yan Xie jiang－hai
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•273•
实 验 研 究
Experimental study
（Chinese Medicine Hospital of Yunnan Province Kunming, Yunnan, 650021）
Abstract Objective: The research is trying to observe the influence of Turbidity-reducing Granule on α- ***A
of Rats with five sixes kidneys cut with chronic renal failure so as to observe the mechanism of the granule in its
therapy function for CRF and its resistance mechanism of renal interstitial fibrosis. Methods: 5 / 6 subtotal
nephrectomy method (two surgeries) to establish CFR rats. Rats were Fed administered or saline 60 consecutive
days. Immunohistochemical staining (SP method) were used to detect α-***A expression.Results:
Turbidity-reducing Granule can reduce the experimental rat kidney tissue α-***A expression， and had
significant difference with model control group (P <0.05), There was no significance between
Turbidity-reducing Granule group and benazepril control group (P>0.05).Conclusion: It is concluded that
Turbidity-reducing Granule can prevent the express of rat kidney’s α-smooth muscle antibody; In addition it can
interfere with the interstitial fibrosis of kidney and prolong the progress of rat’s CRF.For this,,and its effect can
match that of Benazepril.
Key Words Turbidity-reducing Granule; Chronic renal failure; Renal interstitial fibroblast; α-smooth muscle
antibody.
（云南省中医医院，云南昆明，650021）
摘要 目的：观察降浊颗粒对5/6 肾切除慢性肾衰竭大鼠肾组织α- 平滑肌肌动蛋白（α- ***A)的影响，以
观察降浊颗粒对慢性肾衰竭(CRF)的治疗作用及抗肾间质纤维化的机理。方法： 5/6 肾大部切除法（两期
手术）建立CFR 大鼠模型。动物灌胃给生理盐水或给药连续60 天。取大鼠肾脏病理切片，采用免疫组织
化学染色（SP 法）法检测α-***A 表达情况。结果：降浊颗粒能够降低实验大鼠肾组织中的α-***A 的表
达，与模型对照组比较差异有显著性意义（P<0.05），与贝那普利对照组比较差异无显著性（P>0.05）。
结论：降浊颗粒可以抑制大鼠肾组织α-***A 的表达，干预肾间质纤维化，从而延缓大鼠CRF 进展，其疗
效与贝那普利相当。
关键词 降浊颗粒；慢性肾衰竭； 肾间质纤维化；α- 平滑肌肌动蛋白
The Influence of Turbidity-reducing Granule on α- ***A of Rats with Chronic Renal Failure
Ji qin Li xiao Yang wen－rong Gu yan Xie jiang－hai
第九届世界中医药大会
The 9th World Congress of Chinese Medicine
•273•
实 验 研 究
Experimental study
（Chinese Medicine Hospital of Yunnan Province Kunming, Yunnan, 650021）
Abstract Objective: The research is trying to observe the influence of Turbidity-reducing Granule on α- ***A
of Rats with five sixes kidneys cut with chronic renal failure so as to observe the mechanism of the granule in its
therapy function for CRF and its resistance mechanism of renal interstitial fibrosis. Methods: 5 / 6 subtotal
nephrectomy method (two surgeries) to establish CFR rats. Rats were Fed administered or saline 60 consecutive
days. Immunohistochemical staining (SP method) were used to detect α-***A expression.Results:
Turbidity-reducing Granule can reduce the experimental rat kidney tissue α-***A expression， and had
significant difference with model control group (P <0.05), There was no significance between
Turbidity-reducing Granule group and benazepril control group (P>0.05).Conclusion: It is concluded that
Turbidity-reducing Granule can prevent the express of rat kidney’s α-smooth muscle antibody; In addition it can
interfere with the interstitial fibrosis of kidney and prolong the progress of rat’s CRF.For this,,and its effect can
match that of Benazepril.
Key Words Turbidity-reducing Granule; Chronic renal failure; Renal interstitial fibroblast; α-smooth muscle
antibody.
